产品货号：
Z39432
中文名称：
ZO-1/TJP1抗体
英文名称：
Anti-ZO-1/TJP1 Antibody (Clone#3E12)
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-ZO-1/TJP1 Antibody (Clone#3E12) |
| 指标别名 | Tight junction protein 1;Tight junction protein ZO 1;TJP1;ZO 1;ZO1;ZO-1;Zona occludens protein 1;Zonula occludens protein 1 |
| 克隆性 | Monoclonal |
| 检验物种 | human |
| 应用范围 | WB,IHC,ICC/IF,FCM |
| 基因名称 | TJP1 |
| 克隆号 | 3E12 |
| 抗体来源 | Mouse |
| 抗体类型 | IgG1 |
| 免疫原 | E.coli-derived human TJP1 recombinant protein (Position:H1178-F1527).Human TJP1 shares 82% amino acid (aa) sequence identity with mouse TJP1. |
| 实际分子量 | 220KD |
| 成分 | 200μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 200μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | Tight junction protein ZO-1 is a protein that in humans is encoded by the TJP1 gene.It is mapped to 15q13.1.This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions.The encoded protein may be involved in signal transduction at cell–cell junctions.It has been found that injected CagA associates with the epithelial tight-junction scaffolding protein TJP1 and the transmembrane protein junctional adhesion molecule,causing an ectopic assembly of tight junction components at sites of bacterial attachment,and altering the composition and function of the apical-junctional complex. |
| Uniprot ID | Q07157 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (ZN1928) for Western blot,and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (ZN1866) for IHC(P) and ICC. |
| 推荐稀释比 | Western blot (WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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